中文 | ENG

第57卷 第2期 2024-3
Necrotizing fasciitis resulted from foreign body ingestion and intestinal perforation in an incisional hernia: Report of a case

.........................

第57卷 第2期 2024-3
Application of artificial intelligence in endodontic microsurgery

.........................

第57卷 第2期 2024-3
A rare case of cerebral metastasis of malignant peripheral nerve sheath tumor in a patient with neurofibromatosis type 1

.........................

第57卷 第2期 2024-3
Ethic in commenting and clinical surgery journal publication: Emerging issue in the era of social media—A story from “Beall” to “Jaime” and independent researcher

.........................

第57卷 第2期 2024-3
Ophidascaris robertsi, the new emerging human parasite and neurosurgery

.........................

第57卷 第2期 2024-3
Tetanus toxoid IgG, usefulness in clinical surgery, and diagnostic property limitation

.........................

第57卷 第2期 2024-3
Deaths among surgeons while on duty: An important issue in surgery and occupational medicine that should not be disregarded

.........................

第57卷 第2期 2024-3
ChatGPT and its use in clinical surgery: A comment on its pro and con

.........................

第57卷 第2期 2024-3
Isolated spinal neurocysticercosis and pseudotumor cerebri: Tropical problem to be noted

.........................

第57卷 第2期 2024-3
Management of massive hemothorax resulting from traumatic diaphragm rupture with kidney avulsion injury by combined anterior thoracoabdominal approaches

.........................

第57卷 第2期 2024-3
Laparoscopic partial splenectomy for sclerosing angiomatoid nodular transformation: A case report and literature review

.........................

第57卷 第2期 2024-3
Using three-dimensional versus two-dimensional laparoscopy in sleeve gastrectomy: A case matched comparison

.........................

第57卷 第2期 2024-3
Effectiveness of placing prophylactic mesh with the Sugarbaker technique via laparoscopic abdominoperineal resection for rectal cancer: 1-year results

.........................

第57卷 第2期 2024-3
Nylon cable ties–assisted delay primary closure of fasciotomy wound in patients of forearm compartment syndrome

.........................

第57卷 第2期 2024-3
Prefabricated venous fl ap, an advanced free venous fl ap for distant transfer: An experimental rat study

.........................

第57卷 第1期 2024-1
Isolated lower leg monoplegia due to traumatic intraparenchymal hemorrhage

.........................

第57卷 第1期 2024-1
Reflections on the association between cholecystectomy, cholelithiasis, and colorectal cancer

.........................

第57卷 第1期 2024-1
Molluscum contagiosum, false positive, and incidence: Concern in surgery

.........................

第57卷 第1期 2024-1
New form of COVID-19 vaccine: A new advanced technology for fighting the remained problem

.........................

第57卷 第1期 2024-1
COVID-19 vaccination, acute appendicitis, interrelationship, and jointed probability: A concern

.........................
登入帳號才能閱讀全文
 
篇名 Role of mitochondrial DNA copy number alteration in nonsmall cell lung cancer
作者 Chen‑Sung Lin, Yi‑Chen Yeh, Siao‑Cian Pan, Shih‑Yu Lu, Yann‑Jang Chen, Wen‑Yu Chueh, Yau‑Huei Wei
卷期/出版年月 53卷5期 (2020/10)
頁次 165-176
摘要 Background: Mitochondrial dysfunction may involve in the progression of human non-small cell lung cancers (NSCLCs). We analyzed the mitochondrial DNA (mtDNA) copy number, the expression levels of mitochondrial biogenesis‑related proteins including pyruvate dehydrogenase, mitochondrial transcription factor A (TFAM) and mtDNA‑encoded peptide NADH dehydrogenase subunit 1 (ND1), and the expression level of hexokinase II (HK-II) in human NSCLCs both ex vivo and in vitro. Materials and Methods: Paired cancerous and non‑cancerous pathological specimens from 20 resected NSCLCs and an NSCLC cell line, the H23, were used in this study. H23 was infected by lentiviral particles to knockdown (KD) the expression of TFAM. TFAM‑Null and TFAM‑KD represent the control and TFAM knocked‑down H23 cells, respectively. The mtDNA copy number was measured by quantitative real‑time polymerase chain reaction and the protein expression levels were measured by immunohistochemical staining and Western blotting, respectively. Results: Low TFAM expression (P = 0.066) and low mtDNA copy number of NSCLCs (P = 0.009) were poor prognostic variables in NSCLC patients. Advanced T4 NSCLCs had lower TFAM expression (P = 0.021), lower expression of mtDNA‑encoded ND1 polypeptide (P = 0.049), and lower mtDNA copy number (P = 0.050) than did T1 or T2/T3 NSCLCs, respectively. TFAM‑KD cells expressed lower levels of TFAM protein (P < 0.005), ND1 polypeptide (P < 0.005) and mtDNA copy number (P = 0.003), but higher level of vimentin protein (P = 0.045) and higher transwell migration activity (P = 0.003) than did TFAM‑Null cells. Conclusion: Mitochondrial dysfunction caused by lower levels of TFAM, mtDNA copy number, and mtDNA‑encoded ND1 polypeptide may play an important role in the progression of NSCLCs.
關鍵詞 Mitochondrial DNA copy number, mitochondrial transcription factor A, non-small cell lung cancer, prognosis, transwell migration
分類 Original Article

台灣外科醫學會雜誌 © 2006 Taiwan Surgical Association All Rights Reserved
會 址:台北市南京東路五段 31 號 3 樓
電 話:(02) 2769-7845 傳 真:(02) 2746-7149 Email: journal@24drs.com