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| 篇名 |
Salt-modified Polycaprolactone Collagen Scaffold for |
| 作者 |
Dah-Shyong Yu, Hong-I Chen, Sun-Yran Chang, Cheng-Ping Ma |
| 卷期/出版年月 |
40卷6期 (2007/12) |
| 頁次 |
229-235 |
| 摘要 |
Objective: A synthetic polymer, polycaprolactone (PCL) scaffold, was designed in order to enhance its cell affinity, porosity, and biocompatibility in performing tissue engineering.
Methods: The salt-modified PCL-collagen copolymer was synthesized by a dioxane
polymerization method with different weight ratio of salt and coated by collagen. Scaffolds with various thickness were synthesized and dried in a Teflon plate. The characteristics of these synthetic scaffolds were analyzed with regard to morphological, biomechanical and biocompatibilitic properties. Cellular growth and morphology were observed under an inverted microscope and a scanning electron microscope. The proliferation rate of cultured cells was determined using a microculture tetrazolium method. The confirmation of cultured cells was performed through several aspects using specific marker stains. The biodegradability of scaffolds was examined after long term soaking in the medium.
Results: The copolymer was very flexible and elastic, and they could maintain their high elasticity under cyclic mechanical strain conditions. The highly porous scaffold withstood for an initial 1 week without any deformation and sustained for at least 3 weeks in culture media without prominent degradation. When 1:4 wt% salt was used to modify the PCL-collagen scaffold, the amount of adhesion of the fibroblasts and cultured urothelial cells on the scaffolds dramatically increased when compared with the control PLC alone. The taking and growth of cells were observed at 2 days interval and continued in proliferation of cells for 3 weeks. The cell adhesion and proliferation on the scaffolds of various pore-size were increased with increasing pore size.
Conclusions: These results indicate that the salt-modified PCL-collagen copolymer scaffolds are feasible for urothelial proliferation. The improvement may be helpful for the extensive applications of PCL-salt-collagen copolymer scaffold in urological tissue engineering. |
| 關鍵詞 |
Scaffold, salt modified PCL-collagen copolymer, urothelial cells, tissue |
| 分類 |
Original Article |
|
