| 摘要 |
This experiment was designed to investigate the effect of portal vein ligation on liver regeneration after hepatectomy, since liver blood flow, especially of portal blood, is considered important for liver regeneration. The male Wistar rats used in this study were divided into two groups. The rats of the control group underwent Higgins’ partial hepatectomy. In addition to partial hepatectomy, the branch of the portal vein to the caudate lobe of the remaining liver was ligated in the experimental group. Remnant liver weight, DNA synthesis, autoradiogram and mitotic figures were used as markers of regeneration and measured at 12h, then 1, 2, 3, 5, 7 and 14 days after hepatectomy. The remnant liver of the control group regained its original weight at about two weeks after hepatectomy. An increase of DNA synthesis was reached at the 3rd day; a slow decrease was then followed. A peak response of autoradiogram was also reached at the 3rd day; then the response was not so prominent in the following days. Mitotic figures were most remarkable at the 3rd day. Such regenerative activities were almost inconspicuous, but not completely absent, in the caudate lobe of the experimental rats in which the branch of the portal vein was ligated. On the contrary, the regenerative responses were noted earlier and more obviously in the remnant liver excluding the caudate lobe in the experimental groups than for the control group, e.g. DNA synthesis reached its peak at the 1st day, autoradiogram had a maximum response at the 1st day, and the mitotic figures were most prominent at the 2nd day; the remnant liver also regained its weight at 2 weeks. It was concluded that deprivation of portal
blood flow does not completely abrogate liver regeneration after hepatectomy, but it does cause some delay in regeneration. |
