台灣外科醫學會雜誌

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篇名	Effect in vitro of Subeschar Fluid on Amino Acid Uptake in Skeletal Muscle after Burn Injury
作者	Yu-Wen Tang
卷期/出版年月	29卷6期 (1996/12)
頁次	461-467
摘要	Inhibition of amino acid transport in skeletal muscle after thermal injury has been demonstrated previously. The present study investigated the effects, in vitro, of subeschar fluid from burned victims on amino acid trans-port in incubated soleus muscles. Subeschar fluid was collected from just underneath the full thickness of the burn area. Since burn injury may induce increased capillary permeability, the subeschar fluid was believed to transude from the capillaries and to contain high level"burn skin toxin Thirteen patients with severe thermal injury (35% to 90% total body surface area) entered this study. Subeschar fluid was collected in several proce-dures, beginning as early as 18 hours after injury and continuing for 4 to 8 hours. Amino acid transport was studied with 'H-y-aminoisobutyric acid (c-AIB). Subeschar fluid was added in vitro to incubated soleus muscles from healthy animals. Amino acid transport was measured by determining intracellular uptake of x-AIB during a 2h incubation. x-AIB uptake was reduced significantly when subeschar fluid was added to incubated muscle. This suppressive effect increased following either burn area increase or the time interval of subeschar fluid collection after injury. A factor or factor(s) exists in subeschar fluid which depressed muscle amino acid transport, postburn.
關鍵詞	amino acids uptake, skeletal muscle, burns, subeschar fluid
分類	Original Article