| 摘要 |
In study the Wiggers shock model was used to investigate the effect of the intestines on hepatic adenosine triphosphate (ATP) levels after hemorrhagic shock. Rats were subjected to laparotomy for the removal of the entire small and large intestines (experimental group) and sham opera-tion (control group) prior to bloodshedding. During the period of shock, the animals were maintained at 30-35 mmHg arterial pressure for two hours. After reinfusion of the shed blood, survival data were recorded for three hours and liver tissues were sampled from the survivors. The lethality rates were 41% (5/12) and 0% (0/13) for the sham-enterectomized and enterec-tomized groups, respectively (P<0.05). Measurement of hepatic ATP by the luminometric method showed that hepatic ATP contents were signifi-cantly reduced in both the experimental and control groups after shock (p's <0.05). However, hepatic ATP depletion of greater magnitude occurred in
the control group; significantly lower amounts of ATP were demonstrated in the liver tissues of the sham-enterectomized group (367+95 nmol/gm, mean+SEM, n='7) than in that of the enterectomized group (870土100 nmol/gm, mean+SEM, n=13) at 5 hours postshock (p<0.05). Conclusions: These experimental findings show that in the absence of the intestines, hemorrhagic shock is associated with both an improved survival outcome and higher hepatic ATP levels in rats, suggesting the importance of intestinal participation in process leading to hepatic ATP depletion and irreversibility in shock. |
