| 摘要 |
This study was aimed at investigating hemorrhage-induced tumor necrosis factor-a (TNF-a) secretion in the animal model lacking the intestines. . A Wiggers model of hemorrhagic shock (30-35 mm Hg for 120 minutes) and restoraton of the shed blood was used. After enterectomy, the experimen- tal group and control group animals were subjected to hemorrhagic shock and sham shock, respectively. The average volume (mean+SEM) of shed blood was 6.83-0.35 mL in experimental animals, which accounts for 29.8+1.4% of blood volume. After shock, mean arterial pressure (mean+SEM) recovered to 79.7-2.84 mm Hg in the experimental group (n=17), which was of no significant difference from that of the control group (87.2+2.43
mmHg, n=15; p>0.05). In the experimental group, TNF-o concentrations in
systemic circulation, quantitated by enzyme-linked immunosorbent assay,
were found to be significantly elevated at 60 minutes (95.8-32.9 pg/mL,
p<0.01) post-shock and peak at two hours (410.2-36.0 pg/mL, p<0.001) after restoration of the shed blood. TNF-a molecules remained undetectable in the blood of sham-shock group throughout the experiment. Limulu assay used to detect endotoxins in blood samples showed no detectable endotoxemia in any specimen from each group. Conclusions: It was concluded that removal of the intestines does not prevent hemorrhage-induced TNF-d secretion, implying a gut-unrelated mechanism of TNF-c formation in hemorrhagic shock. |
