| 摘要 |
An ideal animal model for spontaneous metastasis of human lung cancer may provide better insight into the growth, progression, and metastasis of cancer cells, as well as an opportunity to develop methods for its control. Five human lung cancer cell lines (SK-LU-1, Calu-1, H2981, NCI-Hut 125, and CH-27 LC-1) were selected in an attempt to develop such a model. The tumorigenic potential of these cells was initially assessed by their ability to form colonies in semisolid soft agar (0.33%). Subsequently, SCID mice were inoculated with these cell lines through three different routes, i.e. intra-bronchial (i.b.) instillation, percutaneous intrathoracic (p.t.), and subcuta-neous (s.c.), and the results were evaluated. Human adenocarcinoma cell line H2981 formed colonies in semisolid soft agar and correspondly, showed higher tumorigenic potential in SCID mice. The tumors formed by s.c. im-plantation were encapsulated and led to no metastasis. The percentage of tumor formation of five human lung cancer cell lines in SCID mice with p.t. implantation were 60% (3/5) for H2981, 40% (2/5) for NCI-Hut 125 and no growth for the other three cell lines. Extensive pleural and chest wall in-volvements without lung parenchyma lesion or metastasis resulted from p.t. implantation. On the contrary, the mice inoculated through the i.b. route had lung parenchyma tumors and regional mediastinal lymph node meta-stases were noted in 66% (4/6) of tumor-bearing mice. In conclusion, in-
oculation of SCID mice through the i.b. route stimulates tumor formation similar to lung cancer formation in human patients. Thus, this method is useful for in vivo studies of spontaneous metastatic biology and experimen-tal therapeutics for advanced lung cancer. |
