| 篇名 |
Heparin Immobilization onto Polyurethane Surfaces and Their Characterization |
| 作者 |
Shoei-Shen Wang, Yeong-Shang Lin, Nai-Kuan Chou, Shu-Hsun Chu |
| 卷期/出版年月 |
31卷6期 (1998/12) |
| 頁次 |
351-361 |
| 摘要 |
Polyurethane (PU) has been widely used as biomaterial in artificial heart and
vascular graft applications and reveals effective blood compatibility. However, PU
still does not show excellent blood compatibility because fibrin formation is
evident after in vivo evaluation. In this paper, heparin was covalently immobilized
onto the surface of PU to increase the biocompatibility.
Both photochemical method and traditional chemical bonding were used to couple
heparin onto PU surfaces to decrease heparin release from the surfaces and finally
to maintain the activity of immobilized heparin molecules. The basic procedures
for photochemical immobilization were as follows. First, heparin reacted with
phenyl azide to become heparin-azide; polyethylene glycol (PEG) was coupled
onto the PU surface to become PU-PEG respectively. Second, by photochemical
reaction, heparin-azide (photohep) was immobilized onto the PU-PEG surfaces
and became a PU-PEG-photohep surface structure.
Our results showed that heparin was successfully coupled onto PU-hexamethylene
diisocyanate (HMDI)-polyethyleneimine (PEI)- PEG-photohep and PU-HMDI-
PEG-photohep via photochemical method, and PU-HMDI-PEG-HMDI-heparin
surfaces via traditional chemical bonding. From the results of the sulfur and
carbon signals by Electron Spectroscopy for Chemical Analysis (ESCA) and
heparin quantitative analysis, we found that photochemical method immobilized
more heparin on PU surfaces, and PEI could greatly enhance the photochemically
immobilized heparin density. |
| 關鍵詞 |
polyurethane, heparin, photochemical reaction, polyethyl-eneimine, blood compatibility |
| 分類 |
Original Articles |
