| 篇名 |
Effect of 5-Fluorouracil on N-Acetyltransferase Activity in Colon Cancer Tissues |
| 作者 |
Chi-Fu Hung, Jin-Gung Chung, Kau-Chi Ku, Jen-Liao |
| 卷期/出版年月 |
32卷6期 (1999/12) |
| 頁次 |
245-251 |
| 摘要 |
Human epidemiological studies suggest an association between N-acetyltransferase (NAT) and colon cancer. Arylamine N-acetylation capacity of the human colon mucosa, catalyzed by acetyl coenzyme A-dependent N-acetyltransferase, may be an important causative factor in the inititation of colorectal cancer. Human healthy colon mucosa and colon cancerous cytosol (subcellular fraction) from 40 fresh surgical samples were investigated for the effect of 5-fluorouracil (5-FU) on the activity of NAT toward p-aminiobenzoic acid and 2-amniofluorene using an acetyl coenzyme A (AcCoA) recycling assay and high pressure liquid chromatography (HPLC). 5-FU is a cancer chemotherapeutic agent used in the treatment of colon cancerous cytosol is found to be inhibited by 5-FU. Its presence in the assay medium shows an inhibition in PABA-NAT activity about of 23.4% and 50.5% in healthy colon mucosa (p<0.05), 48.3% and 73.8% in cancerous colon tissues (p<0.02) at 1mM and 2mM of 5-FU concentrations respectively; while as AF-NAT activity decreased about 21.2% and 55.1% in healthy colon mucosa (p<0.05), 42.2% and 68.4% in cancerous colon tissues (p<0.02) with 1mM and 2mM of 5-FU concentrations respectively.
The results of this study show that NAT activity in colon cancerous tissue cytosol is higher than that in healthy colon mucosa cytosol, and 5-FU can inhibit NAT activity in healthy colon mucosa as well as in colon cancerous tissue cytosol which can be attributed to its anticancer activity of 5-FU. |
| 關鍵詞 |
arylamin, colon cancer, 5-fluorouracil, N-acetyltransferase. |
| 分類 |
Original Articles |
