| 摘要 |
We studied the effect of ascorbic acid (vitamin C) on plasma amino acid transport in rats for 7 days following a 40% trid-degree burn injury. Glutamine, leucine and alanine were tested to monitor the plasma amino acid profile. Rats with burns were fluid-resuscitated with intraperitoneal Ringer's lactate solution according to the Parkland formula (4cc/Kg/%burn). The rats were divided into five groups: nonburn without treatment, nonburn with vitamin C treatment (0.4mg/Kg/day), burn without treatment, burn with vitamin C treatment (0.4mg/Kg/day) and burn with vitamin C treatment (0.6mg/Kg/day). Blood samples were taken daily for 7 days (8 times) from the tail vessel for amino acid assays by HPLC. The nonburn groups showed no significant differences in gultamine, leucine or alanine levels. In the burn group with no vitamin C treatment, glutamine decreased and leucine and alanine increased. However, the only significant increasese were noted int the vitamin C-treated (0.4mg/Kg/day) burn group when compared with the other four groups on the 4-7th days postburn. Our results suggest that vitamin C can increase plasma amino acid profile (glutamine, leucine and alanine) after the third day postburn. Large amounts of vitamin C (0.6mg/Kg/day) did not have any added benefit over ordinary dose vitamin C(0.4mg/Kg/day) did not have any added benefit over ordinary dose vitamin C(0.4mg/Kg/day). We conclude that ordinary dose vitamin C adminstered early posburn increases plasma amino acid levels. |
