| 摘要 |
Objective: The prognosis of patients suffering from oral cancer can be improved by
early diagnosis. Exact demarcation of tumor margins could contribute to optimal
results in surgical excision and reconstruction. Therefore, this study evaluates
Photofrin® with protoporphyrin IX (PPXI) fluorescence as a new diagnostic procedure
for the detection of oral neoplasms in animal models. The aim of this study is
to assess these parameters, which can be extended to clinical applications for human
oral neoplasms.
Methods: Fourteen male Golden Syrian hamsters were included in this study. 0.5%
D.M.B.A (9,10 dimethyl1-1, 2-benzanethracene), was brushed onto the cheek pouches
bilaterally daily for 2 weeks. Hamsters with oral neoplasms received 2.5mg/kg
Photofrin® intravenously. After a period of 3 hours, the neoplasms underwent fluorescence
illumination ( ex = 380-420 nm). A quantitative analysis of the fluorescence
contrast between the neoplastic and surrounding tissues was performed using the
RGB Mode (Red, Green, Blue) and the GS (Gray Scale). Statistical analysis was performed
by means of ANOVA test for multiple comparisons.
Results: Analysis of the 14 hamsters 28 biopsies revealed that 4 (14%) displayed
squamous hyperplasia (1 mild, 3 severe) and 24 (86%) displayed squamous cell carcinoma.
The sensitivity of neoplastic tissue evaluated using the RGB and GS modes
combined reached 92% in the macroscopic study, and 93% in the microscopic study.
The specificity of neoplastic tissue evaluated using the RGB and GS modes combined
reached 95% in the macroscopic study, and 97% in the microscopic study. The difference
between healthy tissue and the lesions as a group is statistically significant.
Conclusions: Light-induced fluorescence detection using Photofrin® provides a sensitive
technique for the early identification of malignant neoplasms in the oral cavity
of our animal models. |
