| 篇名 |
Photosensitizing Capabilities of Photofrin in Vascular Endothelial Cells |
| 作者 |
Cheng-Jen Chang, Shwu-Fan Ma, Fu-chan Wei |
| 卷期/出版年月 |
34卷6期 (2001/12) |
| 頁次 |
287-293 |
| 摘要 |
The object of the present study was to evalutate the feastibility of photodynamic therapy (PDT) using Photofrin (porfimer sodium), an United States Food and Drug Administration approved photosensitizer. The in vitro absorption and photosensitizing activities of Photofrin were evaluated with cultured human microvascular endothelial cells and in vivo activities were evaluated with ductal carcinoma cell stimulated mammary tumor. The viability of microvascular endothelial cells was assessed using rhodamine-phallodin coupled α-tubulin antibody specific for cytoplasmic microtubule and visualized by immunofluore scence microscopy. At the optinal wavelength of 630 nm, the percentage of microvascular endothelial cells killed by variable concertrations of Photofrin was measured by either live/dead or lactate dehydrogenase-released assays and the in vivobiological activity was assessed by determining the amount of necrosis prodused in stimulated mammary tumor. A combination of 3.5 ug/mL Photofrin and laser light at 630 nm with a powerdensity of 100 mW/cm2 resulted in a 20% cell kill. The lactate dehydrogenase released from microvascular endothelial cells after PDT showed a 50% optical density obtained from each concentration of photosensitizer of 50% cell killing after laser illumination. Stained histology specimens of stimulated mammary rumor demonstrated significant vascular destruction 1 hour after laser illumination. Stained histology specimens of stimulated mammary tumor demonstrated significant vascular destruction 1 hour after PDT. In conclusion, Photofrin has the capabilities to destroy microvascular endothelial cells in vitro and vasculature in vivo. Hower, the degree of absorption and tissue destruction in vasculature in vivo and defferent anatomical structures should be considered and will be included in our subseqent studies. |
| 關鍵詞 |
Photofrin, microvascular endothelial cell, chicken bombs |
| 分類 |
Original Articles |
